Presentation Title

Developing Growth Promotion Strategies for Cressa truxillensis and Distichlis spicata to Improve Success of Degraded Wetland Restoration in Los Angeles, CA.

Faculty Mentor

Dr. Michelle Lum

Start Date

23-11-2019 10:45 AM

End Date

23-11-2019 11:30 AM

Location

66

Session

poster 4

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

With a goal to restore a degraded area of the Ballona Wetlands Ecological Reserve in Los Angeles, a revegetation and seed collection project was developed with an interest in looking at how the microbial community could benefit restoration. Certain rhizobacteria have the ability to improve growth of plants(Plant Growth Promoting Rhizobacteria), and can be found on and inside the roots of plants. This study in particular focused on improving revegetation success of Cressa truxillensis (alkali weed) and Distichlis spicata (saltgrass). Seeds from alkali weed and saltgrass rhizomes were collected to isolate bacteria and determine suitable growth conditions as well as scarification methods for the seeds. Mechanical scarification and moderate saline environments produced optimal growth conditions for the plants. Following isolation, strains were characterized by morphology, biochemical assays, and inoculated onto seeds and plants to quantify the strains’ ability to promote plant growth. 16S rRNA sequencing was used to identify strains from the seeds of alkali weed as Paenibacillus spp., a known plant PGPR, and strains from the roots of alkali weed and saltgrass as Bacillus sp. and Pseudomonas spp., respectively. Strains identified were found to be salt tolerant and have other properties including nitrogen fixation and phosphorus solubilization. Next steps will be transplanting the inoculated plugs into the wetland area along transects and additional studies to determine how the microbial community changes across multiple inoculations. Results from this study are directly informing adaptive management strategies for improving wetland health.

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Nov 23rd, 10:45 AM Nov 23rd, 11:30 AM

Developing Growth Promotion Strategies for Cressa truxillensis and Distichlis spicata to Improve Success of Degraded Wetland Restoration in Los Angeles, CA.

66

With a goal to restore a degraded area of the Ballona Wetlands Ecological Reserve in Los Angeles, a revegetation and seed collection project was developed with an interest in looking at how the microbial community could benefit restoration. Certain rhizobacteria have the ability to improve growth of plants(Plant Growth Promoting Rhizobacteria), and can be found on and inside the roots of plants. This study in particular focused on improving revegetation success of Cressa truxillensis (alkali weed) and Distichlis spicata (saltgrass). Seeds from alkali weed and saltgrass rhizomes were collected to isolate bacteria and determine suitable growth conditions as well as scarification methods for the seeds. Mechanical scarification and moderate saline environments produced optimal growth conditions for the plants. Following isolation, strains were characterized by morphology, biochemical assays, and inoculated onto seeds and plants to quantify the strains’ ability to promote plant growth. 16S rRNA sequencing was used to identify strains from the seeds of alkali weed as Paenibacillus spp., a known plant PGPR, and strains from the roots of alkali weed and saltgrass as Bacillus sp. and Pseudomonas spp., respectively. Strains identified were found to be salt tolerant and have other properties including nitrogen fixation and phosphorus solubilization. Next steps will be transplanting the inoculated plugs into the wetland area along transects and additional studies to determine how the microbial community changes across multiple inoculations. Results from this study are directly informing adaptive management strategies for improving wetland health.