Presentation Title

Comparing the Oncolytic Properties of a Chimeric Poxvirus CF33 with Herpes Simplex Virus Oncovex

Faculty Mentor

Yuman Fong, Shyambabu Chaurasiya

Start Date

23-11-2019 10:45 AM

End Date

23-11-2019 11:30 AM

Location

84

Session

poster 4

Type of Presentation

Poster

Subject Area

biological_agricultural_sciences

Abstract

Oncolytic viruses can selectively replicate in and kill cancer cells; one such virus, CF33, is a chimeric poxvirus that has shown oncolytic promise in preclinical studies. In this study we investigated whether CF33 could super-infect cells, i.e., whether a cell can be infected by more than one virus particles. Ability of CF33 to super-infect cells would allow us to use the virus with different modifications in combination, which may result in better therapeutic index. To investigate the potential of CF33 to super-infect cells, we used CF33 encoding GFP and mCherry to co-infect CV-1 cells. We also studied if the poxvirus CF33 and the herpes virus Oncovex could co-infect cells. Our data show that CF33 virus can super-infect CV-1 cells with high frequency; however, although CF33 and Oncovex could co-infect CV-1 cells, the frequency at which this occurs is very low. Cytotoxicity results of the two viruses with human cancer cell lines, MIA PaCa-2 and AGS, indicated the cell lines to be resistant to Oncovex, but able to be killed dose-dependently by CF33.

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Nov 23rd, 10:45 AM Nov 23rd, 11:30 AM

Comparing the Oncolytic Properties of a Chimeric Poxvirus CF33 with Herpes Simplex Virus Oncovex

84

Oncolytic viruses can selectively replicate in and kill cancer cells; one such virus, CF33, is a chimeric poxvirus that has shown oncolytic promise in preclinical studies. In this study we investigated whether CF33 could super-infect cells, i.e., whether a cell can be infected by more than one virus particles. Ability of CF33 to super-infect cells would allow us to use the virus with different modifications in combination, which may result in better therapeutic index. To investigate the potential of CF33 to super-infect cells, we used CF33 encoding GFP and mCherry to co-infect CV-1 cells. We also studied if the poxvirus CF33 and the herpes virus Oncovex could co-infect cells. Our data show that CF33 virus can super-infect CV-1 cells with high frequency; however, although CF33 and Oncovex could co-infect CV-1 cells, the frequency at which this occurs is very low. Cytotoxicity results of the two viruses with human cancer cell lines, MIA PaCa-2 and AGS, indicated the cell lines to be resistant to Oncovex, but able to be killed dose-dependently by CF33.