Presentation Title

The Upregulation of Cell Viability via Probiotics

Presenter Information

Hanien SamaraFollow

Faculty Mentor

Dr. Jerome Garcia

Start Date

23-11-2019 10:45 AM

End Date

23-11-2019 11:30 AM

Location

182

Session

poster 4

Type of Presentation

Poster

Subject Area

health_nutrition_clinical_science

Abstract

The Upregulation of Cell Viability via Probiotics

Abstract:

Probiotics encompass a variety of microorganisms that are essential to the maintenance of human homeostasis. Current research emphasizes the positive impact probiotic coagulants have upon the gastrointestinal tract with very little research regarding the impact of probiotics on non-digestive cells. Newfound research has been transitioning to studying the investigation of probiotic activity in relation to varying cell types, the mechanism is still unknown with no active component being specified. Prior research within our laboratory revealed increased survivability when cells were pretreated with crude probiotic protein extract and treated with UV Radiation. However, inconsistent production of the probiotic protein hindered the compilation of data. To properly investigate the active components of probiotics, optimal conditions must be present for the consistent precipitation of proteins. Protein precipitation was tested with different conditions such as specific amount (grams) of probiotic kernels administered and the presence of probiotic residue. For example, a sample placed within a clean bottle with no milk layer will reach an optimal PH of 4.7 after 72 hours of fermentation. Having tested and understood such conditions allows our lab to transition into more detailed focuses including protein analysis. Consistent production of the protein allows for research to shift onto the mechanism itself. With preliminary data showing increased cell survival when pretreated with isolated probiotic protein, similar research within our lab hinted at the upregulation of mitochondrial proteins impacting fission and fusion factors and thus cell survival (data not published). As such, western blot analysis of mitochondrial proteins Dynamin-related protein 1 (DRP1) and Mitofusin-2 (MFN-2) were completed, displaying an upregulation of both. With such data, it is thought that the mechanism linking cell survival is linked to mitochondrial function.

Keywords:

- Probiotic

- Mitochondria

- Mechanism

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Nov 23rd, 10:45 AM Nov 23rd, 11:30 AM

The Upregulation of Cell Viability via Probiotics

182

The Upregulation of Cell Viability via Probiotics

Abstract:

Probiotics encompass a variety of microorganisms that are essential to the maintenance of human homeostasis. Current research emphasizes the positive impact probiotic coagulants have upon the gastrointestinal tract with very little research regarding the impact of probiotics on non-digestive cells. Newfound research has been transitioning to studying the investigation of probiotic activity in relation to varying cell types, the mechanism is still unknown with no active component being specified. Prior research within our laboratory revealed increased survivability when cells were pretreated with crude probiotic protein extract and treated with UV Radiation. However, inconsistent production of the probiotic protein hindered the compilation of data. To properly investigate the active components of probiotics, optimal conditions must be present for the consistent precipitation of proteins. Protein precipitation was tested with different conditions such as specific amount (grams) of probiotic kernels administered and the presence of probiotic residue. For example, a sample placed within a clean bottle with no milk layer will reach an optimal PH of 4.7 after 72 hours of fermentation. Having tested and understood such conditions allows our lab to transition into more detailed focuses including protein analysis. Consistent production of the protein allows for research to shift onto the mechanism itself. With preliminary data showing increased cell survival when pretreated with isolated probiotic protein, similar research within our lab hinted at the upregulation of mitochondrial proteins impacting fission and fusion factors and thus cell survival (data not published). As such, western blot analysis of mitochondrial proteins Dynamin-related protein 1 (DRP1) and Mitofusin-2 (MFN-2) were completed, displaying an upregulation of both. With such data, it is thought that the mechanism linking cell survival is linked to mitochondrial function.

Keywords:

- Probiotic

- Mitochondria

- Mechanism